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Electrophoretic and immunofluorescence methods for study of plant cell cultures
Klimešová, Marie ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
In all organisms are rising a reactive oxygen and nitrogen species by the effects of various stress factors and these species have a negative impact on the organism. Due to this species plants have built up an efficient antioxidant system, that helps them to resist negative effects of reactive oxygen and nitrogen species. In this work was researched the effect of hydrogen peroxide and sodium benzoate on the production of hydrogen peroxide, superoxide, reactive nitrogen species and malondialdehyde, contained in the root and above-ground part of maize (Zea mays L.). By use of the fluorescence microscopy there were obtained images of cross-cut of root from which was determined the intensity of fluorescence of individual parts of the root and was examined the effect of the intensity of fluorescence markers of oxidative stress in dependence on the type of the fluorescence filter used.
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Study of plasma activated water influence on soil microorganisms
Lungová, Tereza ; Šimečková,, Jana (referee) ; Krčma, František (advisor)
The bachelor thesis deals with the effects of plasma activated water on microorganisms found in the brown earth sample. The theoretical part is divided into two parts. The first part focuses on the analysis of soil and microorganisms living in it. The second part deals with the general properties of plasma and the formation of plasma-activated water. In the experimental part of this work, plasma-activated water was applied to the leachate of soil microorganisms and also directly to a selected soil sample in various concentrations. Subsequently the effect on the viability of soil microorganisms and the composition of soil microorganisms was observed. Based on the results from the practical part, it was found that plasma-activated water has no significant positive or negative effect on soil microorganisms. For this reason, it will be necessary to focus on specific types of microorganisms in further research.
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Přirozené antioxidanty v potravinách jako prevence oxidativního stresu v organismu
Kaserová, Natálie
The bachelor thesis is focused on natural antioxidants and their fight against oxidative stress in the organism. The first part is about the oxidative stress, what causes it, and its negative or positive effects on the human body. The connection between free radicals and the development of oxidative stress is outlined. The second part of the literature review is devoted to the antioxidants themselves, both natural and natively occurring in the human organism. The low-molecular organic molecules of plants are described in more detail, which exhibit significant antioxidant activity and constitute a substantial part of our diet. The foods or beverages with high content of antioxidant substances are mentioned in the last part of bachelor thesis. This chapter deals with the foods such as vegetables, fruits, nuts and even cereals. Furthermore, from the beverages are presented the tea, coffee, beer, and wine.
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Study of plasma activated water influence on soil microorganisms
Lungová, Tereza ; Šimečková,, Jana (referee) ; Krčma, František (advisor)
The bachelor thesis deals with the effects of plasma activated water on microorganisms found in the brown earth sample. The theoretical part is divided into two parts. The first part focuses on the analysis of soil and microorganisms living in it. The second part deals with the general properties of plasma and the formation of plasma-activated water. In the experimental part of this work, plasma-activated water was applied to the leachate of soil microorganisms and also directly to a selected soil sample in various concentrations. Subsequently the effect on the viability of soil microorganisms and the composition of soil microorganisms was observed. Based on the results from the practical part, it was found that plasma-activated water has no significant positive or negative effect on soil microorganisms. For this reason, it will be necessary to focus on specific types of microorganisms in further research.
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Physiological adaptations associated with body size and longevity in endothermic vertebrates
Pazdera, Lukáš ; Tomášek, Oldřich (advisor) ; Blažek, Radim (referee)
Abstact Lifespan is related to a number of physiological processes that are interconnected. Longevous species are assumed to have evolved certain physiological adaptations that slow down ageing rate and enable long lifespan. The aim of my thesis was to review physiological traits that has been hypothesised to be associated with lifespan, and to evaluate the empirical support for such hypotheses. Studies testing the association of these traits with body size has also been included in my thesis, as body size is known to be tightly related to lifespan. Traits that are reviewed in my thesis due to their hypothesised coevolution with lifespan are as follows: metabolic rate (i.e., a total amount of energy expended by the organism per unit of body mass and unit of time), oxidative stress resistance, telomere length and rate of telomere shortening and intensity of stress and immune responses. Key words: Lifespan, longevity, survival, reproduction, oxidative stress, free radicals, reactive oxygen and nitrogen species, metabnolic rate, immunity, antioxidants
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Electrophoretic and immunofluorescence methods for study of plant cell cultures
Klimešová, Marie ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
In all organisms are rising a reactive oxygen and nitrogen species by the effects of various stress factors and these species have a negative impact on the organism. Due to this species plants have built up an efficient antioxidant system, that helps them to resist negative effects of reactive oxygen and nitrogen species. In this work was researched the effect of hydrogen peroxide and sodium benzoate on the production of hydrogen peroxide, superoxide, reactive nitrogen species and malondialdehyde, contained in the root and above-ground part of maize (Zea mays L.). By use of the fluorescence microscopy there were obtained images of cross-cut of root from which was determined the intensity of fluorescence of individual parts of the root and was examined the effect of the intensity of fluorescence markers of oxidative stress in dependence on the type of the fluorescence filter used.
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Defence of organism against reactive oxide substances and the role of nutrition in it
BENDLOVÁ, Radka
Reactive oxygen and nitrogen species along with environmental toxins are the most frequently cited sources of pathological effects on physiological proceses in man and on the development of degenerative diseases (diseases of civilization). Numerous free radicals commonly occur in the organism because they are part of physiological processes (physical exercive, food processing, the body's immune defence). On one hand highly reactive oxygen species play an important role in antimicrobial protection of the human body, helping to kill microorganismus during phagocytosis. Other side of oxidative stress (overproduction of reactive oxygenates) participates damage to proteins, lipids and nucleic acids. Oxidative damage to biomolecules has to be counteracted by antioxidant defense. Sufficient level of antioxidants in biological tissues act protectively. If we follow the recommended quantity and composition of food rich in antioxidants we can substantially contribute to improving our health and reduce the risk of manifestation of the serious diseases, so called civilization diseases.
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Sources and methods of saturation of the organism with antioxidants
NOVOTNÁ, Nikola
The aim of the thesis is to characterize sources of antioxidants and inquire the depth of knowledge about antioxidants among students of secondary school. The theoretical part of the thesis describes and divides important antioxidants for human health. The main sources of antioxidants in human diet are there divided, described and compared as well. The practical part of the thesis includes the inquiry which evaluates the theoretical interest in antioxidants and dietary intake of antioxidant supplements among 17-19 years-old secondary school students. The results are analyzed and graphically displayed. Research shows that more than a half of the respondents know the term antioxidants and consume these substances in the diet. However, it is important to increase knowledge of antioxidants already among young people and ensure the intake of these essential substances from a varied diet.
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